PCR is based upon ………replication process which is carried out by
DNA polymerase enzyme.
a) in vivo
b) in vitro
c) in bacteria
d) both a and b
Explanation: In vitro is Latin for “in glass.” It describes medical procedures, tests, and experiments that researchers perform outside of a living organism
Which of the following component/s is/are required for carrying out a PCR reaction?
a) template DNA and Taq polymerase
b) primers
c) deoxyribnucleotide tri-phosphate (dNTPs)
d) all of these
Which of the following is not required for a polymerase chain reaction?
a) primer
b) template DNA
c) ddNTPs
d) Taq polymerase
RT-PCR is a method that is used for…
a) amplification of genomic DNA
b) DNA fingerprinting
c) amplification of mRNA sequence
d) analysis of mRNA expression
There are…….types of dNTPs required for polymerase chain reaction.
a) two
b) three
c) four
d) five
Explanation: dATP, dCTP, dGTP, dTTP (d for deoxyribose)
Why primers are required in the process of polymerase chain reaction?
a) to speed up the rate of PCR reaction
b) to make correction
c) DNA polymerase unable to start polymerization
d) all of these
