Taq DNA polymerase is a temperature-tolerant enzyme isolated from……..
a) Thermus aquaticus
b) Haemophilus influenze
c) Staphylococcus aureus
d) Bacillus amyloliquefaciens
Explanation: it a bacterium found in hot springs, catalyze the synthesis of DNA
The two possible ways of cloning a gene are recombinant DNA technology and:
a) gene splicing
b) PCR
c) electrophoresis
d) RFLP
A PCR-amplification cycle consists of ……..basic steps.
a) two
b) three
c) four
d) five
Explanation: Denaturation, Primer annealing and Extension or polymerization
In the denaturation step of PCR, the template is heated to………°C for one minute or up to five minutes.
a) 37
b) 50
c) 63
d) 94
Explanation: at high temperature, the two strands of DNA separated and act as template
If the primer annealing temperature is increased to 94°C, what will happen?
a) extension
b) primer-dimer formation
c) annealing
d) no annealing
How many primers are required for the primer annealing step during PCR?
a) two
b) three
c) four
d) five
Explanation: forward primer and the backward primer
