During annealing step of PCR, what is the standard temperature is required?
a) 37°C
b) 40°C
c) 54°C
d) 94°C
Explanation: the approximate time required for this step is 2 minutes
Which of the following is the final step of polymerase chain reaction?
a) denaturation of template DNA
b) primer annealing
c) extension or polymerization
d) removal of Taq polymerase
What is the optimum temperature for the final step (polymerization) of PCR?
a) 72°C
b) 37°C
c) 94°C
d) 54°C
In how much time, the extension or polymerization step of PCR is
completed?
a) 1 min
b) 2 mins
c) 5 mins
d) 10 mins
A PCR reaction that continues for 30 cycles will produce approximately how many PCR products from a single template DNA molecule?
a) approximately one billion
b) 63
c) approximately one million
d) 128
Explanation: 230= 1073741824
The primer used in polymerase chain reaction has a sequence of bases……
a) 16
b) 12
c) 20
d) 8
