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The collection of bacterial or Bacteriophage clones containing at least one copy of every DNA sequence in a genome of an organism…
a) sonication                        
b) DNA analysis
c) genomic library            
d) DNA fingerprinting

c

A genomic library is a collection of …….clones, containing at least one copy of every DNA sequence in a genome of an organism.
a) bacterial                           
b) Bacteriophage
c) fungal                                               
d) both a and b

d

To construct a genomic library of an organism, how many methods are used?
a) two    
b) three 
c) four   
d) five

b

Explanation: 1. Complete digestion of DNA by restriction enzyme. 2. Without enzyme and 3. By partial enzymatic digestion


Fragmentation of genomic DNA by means of mechanical shearing such as sonication is done by……….
a) restriction enzymes                        
b) without restriction enzymes
c) partial enzymatic digestion          
d) all of these

b

Which of the following is added to locate similar sequences of DNA?
a) cDNA                                               
b) DNA probe
c) mRNA                              
d) polypeptide

b

Explanation: A probe is a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a sample genome.


DNA Sequencing:

DNA sequencing refers to the general laboratory technique for determining the exact sequence of nucleotides, or bases, in a DNA molecule.


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