To understand the complexities of which of the following, DNA sequencing is very essential?
a) gene structure and its expression
b) gene regulation and protein interactions
c) molecular mechanisms of genetic diseases
d) all of these
Which of the following is/are principle/s of any DNA sequencing?
a) to generate piece of DNA of different sizes
b) separation of pieces of DNA by gel electrophoresis
c) reading of sequence from the gel
d) all of these
For generation of different sized DNA fragments, how many different sequencing methods were developed during 1970s?
a) two
b) three
c) four
d) five
Explanation: 1. Sanger’s method and 2. Maxim-Gilbert method
Which of the following DNA sequencing method/s is/are widely used ad similar to the natural process of DNA replication?
a) Sanger’s method
b) Maxim-Gilbert method
c) Automated DNA sequencing
d) both a and b
Explanation: it was developed by Frederick Sanger and Andrew Coulson in 1977. They have awarded Nobel Prize in 1980.
Once the primer is attached to DNA, the solution is divided into ……tubes.
a) two
b) three
c) four
d) five
Explanation: these four tubes are labeled “G”, “A”, “T” and “C”
In Sanger’s DNA sequencing method, “G” tube contains……
a) all four dNTP’s
b) ddGTP
c) DNA polymerase
d) all of these
