Which of the following principle/s is/are different in Automated DNA sequencing than manual sequencing?
a) there is no need of radiolabelling
b) computer have to read the sequence
c) all four types of ddNTPs are used
d) both a and b
Which of the following DNA sequencing method is called as chemical cleavage method?
a) Sanger’s method
b) Maxim-Gilbert method
c) Automated DNA sequencing
d) both a and b
Explanation: it was developed by Allan Maxam and Walter Gilbert in 1976-77.
Which of the following radioactive isotope is used to in Maxam-Gilbert method of DNA sequencing?
a) N15
b) O18
c) P32
d) C14
Explanation: This method is based on nucleobase-specific partial chemical modification of DNA and subsequent cleavage of the DNA backbone at sites adjacent to the modified nucleotides.
Which of the following is added and the labeled DNA samples heated
to 90 °C in Maxam-Gilbert method of DNA sequencing?
a) Tris-Borate-EDTA
b) Tris-Actate-EDTA
c) Dimethylsulphoxide
d) both a and b
DNA Analysis:
DNA profiling (also called DNA fingerprinting and genetic fingerprinting) is the process of determining an individual's deoxyribonucleic acid (DNA) characteristics.
DNA analysis is also known as………..
a) DNA fingerprinting
b) DNA sequencing
c) polymerase chain reaction
d) DNA synthesis
Explanation: this is an examination method that emerged in the 1980s and credited to Alec Jeffrey, an English geneticist
