There are …….possible ways of cloning of gene.
a) two
b) three
c) five
d) many
Explanation: 1. Recombinant DNA technology and 2. Polymerase chain reaction (PCR)
Which of the following gene cloning method is/are in vivo method/s?
a) recombinant DNA technology
b) Polymerase Chain Reaction (PCR)
c) both a and b
d) none of these
Explanation: Recombinant DNA technology involves using enzymes and various laboratory techniques to manipulate and isolate DNA segments of interest.
Recombinant DNA technology involves
a) selection and isolation of desired gene and vector
b) formation recombinant DNA (rDNA)
c) transformation of a suitable host by the recombinant DNA
d) all of these
In order to achieve the task of recombinant DNA technology, which of the following component/s or tool/s is/are required?
a) gene of interest and molecular scissors
b) molecular carrier or vector
c) molecular glue and expression system
d) all of these
Selection and isolation of desired gene or gene of interest can obtained by…….
a) artificial gene synthesis in vitro
b) from mRNA
c) from a chromosomal DNA by DNA scissors
d) all of these
Molecular scissors are………enzymes
a) protease
b) nuclease
c) lipase
d) restriction endonucleases
Explanation: these enzymes cleave the phosphodiester bonds of both strands of DNA
