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DNA polymerase-III cannot initiates replication process, it can add a nucleotide onto only a preexisting……. group/s.
a) 3’ OH                                 
b) 5’ PO4
c) OH of base             
d) both a and b

a



The direction of DNA polymerase-III is from………..
a) 5’ to 3’ end            
b) 3’ to 5’ end
c) both a and b
d) none of them

a

Explanation: The 5' and 3' designations refer to the number of carbon atom in a deoxyribose sugar molecule to which a phosphate group bonds.



Which of the following strands contains Okazaki’s fragments?
a) leading strands       
b) lagging strands
c) template strand      
d) both a and b

b

Explanation: The lagging strand is a single DNA strand that, during DNA replication, is replicated in the 5′ – 3′ direction (opposite direction to the replication fork)



The size of Okazaki’s fragments in the polymerization of lagging strands in prokaryotes is………nucleotides.
a) 10 to 20                  
b) 100 to 200
c) 1000 to 2000          
d) 10000 to 20000

b

The size of Okazaki’s fragments in the polymerization of lagging strands in eukaryotes is………nucleotides.
a) 10 to 20                  
b) 100 to 200
c) 1000 to 2000          
d) 10000 to 20000

c

Termination phase of DNA replication is characterized by………..
a) replacement of primer by DNA nucleotides
b) joining of Okazaki’s fragments in lagging strands
c) attachment of single stranded binding proteins                             
d) both a and b

d

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