Which restriction enzyme makes blunt ends?
a) KpnI
b) BamIII
c) SmaI
d) EcoRI
Naturally restriction enzymes are found in……..
a) human
b) bacteria
c) fungi
d) algae
Explanation: they appear to serve a host-defense role because they chop up and inactivate (restrict) the DNA of infecting viruses
Restriction endonuclease enzymes hydrolyze the……..bond.
a) peptide
b) hydrogen
c) covalent
d) phosphodiester
Explanation: phosphodiester bond is found between deoxyribose sugar and phosphate group
Which of the following act/s as a vehicle/s for carrying foreign
DNA into a host cell for multiplication?
a) vectors
b) clones
c) endonucleases
d) fungus
Explanation: usually small circular DNA molecules of bacterial origin are used as cloning vectors
A DNA molecule should possess which of the following characteristic/s to act as a cloning vector?
a) origin of replication
b) selectable markers
c) multiple cloning sites or polylinker
d) all of these
Which of the following size vectors are more desirable for cloning?
a) large size
b) small size
c) medium size
d) very large size
