Which of the following is a suitable vectorto be incorporated with a large external DNA fragment?
a) large size vector with no origin of replication
b) small size vector
c) small size vector with no origin of replication
d) large size vector
Explanation: because they increase the transformation efficiency and are easy to manipulate
Which of the following vectors are commonly used in recombinant DNA experiments?
a) plasmid-cloning and Bacteriophage-cloning vectors
b) cosmid-cloning vectors and yeast artificial chromosomes
c) bacterial artificial vectors and animal and plant vectors
d) all of these
Which of the following vector is most widely used, versatile and easily manipulated ones?
a) plasmid
b) Bacteriophage vector
c) YACs
d) shuttle vector
Explanation: plasmid is derived most from bacteria
A vector which a capacity of insert DNA size from 250 to 1000kbp is……
a) shuttle
b) BAC
c) YAC
d) all of these
A new vector pBR322 is derived from…
a) plasmid
b) Bacteriophage vector
c) YACs
d) shuttle vector
Explanation: this was first widely used purpose built plasmid vector
The copy number of PBR322 per cell is
a) 10
b) 20
c) 15
d) 30
