The transformation efficiency of PBR322 is very low to the size above gene of choice is…
a) 60kbp
b) 4363
c) 10kbp
d) 1kbp
pBR322 carries…….antibiotic/s gene.
a) ampicillin
b) tetracycline
c) cephalosporin
d) both a and b
If one of the following component is missing bacteria cannot increase the number of its plasmid copies…….
a) ligase enzyme
b) cloning site
c) origin of replication
d) antibiotic resistant gene
Which one of the following enzymes acts as molecular glue in recombinant DNA technology?
a) DNA helicase
b) DNA polymerase
c) DNA ligase
d) restriction endonucleases
Explanation: this enzyme is responsible for the formation of the phosphodiester bone between two adjacent nucleotides and thus joins two double stranded DNA
What will happen if a vector (plasmid) is cut with a different
restriction enzyme which cuts the external DNA to be incorporated in the vector
(plasmid)?
a) cloning
b) no ligation
c) ligation
d) tight ligation
Transformation efficiency is inversely proportional to the size of ……
a) markers
b) carrier
c) vector
d) both b and c
