Transformation can be performed by putting the ……..into the same medium.
a) expression system
b) recombinant plasmid
c) transformed clone
d) both a and b
Bacterial cells take up recombinant plasmid especially if they are treated with ……….…to make them more permeable.
a) sodium chloride
b) calcium chloride
c) magnesium chloride
d) water
Explanation: Calcium chloride helps the DNA to get attracted towards the cell, and hence ensures a higher efficiency of successful transformants.
The transformed clone can be identified by adding a particular…into the medium.
a) acid
b) alkali
c) salt
d) antibiotic
Explanation: that antibiotic for which resistant gene is found in plasmid
Polymerase Chain Reaction:
Polymerase chain reaction (abbreviated PCR) is a laboratory technique for rapidly producing (amplifying) millions to billions of copies of a specific segment of DNA,
The polymerase chain reaction (PCR) is a revolutionary technique in………to amplify of piece of DNA.
a) cell biology
b) biotechnology
c) molecular biology
d) genetics
Explanation: Molecular biology is the study of the molecular underpinnings of the biological phenomena, focusing on molecular synthesis, modification, mechanisms and interactions.
The polymerase chain reaction was originally invented by……….in 1983.
a) Sanger
b) F. Crick
c) Kary Mullis
d) Margulis
Explanation: he was awarded Nobel prize in chemistry in 1993
