Biotechnology                                         

Important concept:
Prokaryotes vs Eukaryotes: What Are the Key Differences? 

Every living organism falls into one of two groups: eukaryotes or prokaryotes. Cellular structure determines which group an organism belongs to. In this article, we will explain in detail what prokaryotes and eukaryotes are and outline the differences between the two.  

Prokaryotes are unicellular organisms that lack membrane-bound structures, the most noteworthy of which is the nucleus. Prokaryotic cells tend to be small, simple cells, measuring around 0.1-5 μm in diameter.   

While prokaryotic cells do not have membrane-bound structures, they do have distinct cellular regions. In prokaryotic cells, DNA bundles together in a region called the nucleoid.

Prokaryotic cell features

Here is a breakdown of what you might find in a prokaryotic bacterial cell.

·    Nucleoid: A central region of the cell that contains its DNA. 

·    Ribosome: Ribosomes are responsible for protein synthesis.

·    Cell wall: The cell wall provides structure and protection from the outside environment. Most bacteria have a rigid cell wall made from carbohydrates and proteins called peptidoglycans.

·    Cell membrane: Every prokaryote has a cell membrane, also known as the plasma membrane, that separates the cell from the outside environment.

·     Capsule: Some bacteria have a layer of carbohydrates that surrounds the cell wall called the capsule. The capsule helps the bacterium attach to surfaces.

·     Fimbriae: Fimbriae are thin, hair-like structures that help with cellular attachment.

·     Pili: Pili are rod-shaped structures involved in multiple roles, including attachment and DNA transfer.

·     Flagella: Flagella are thin, tail-like structures that assist in movement.

Examples of prokaryotes

Bacteria and archaea are the two types of prokaryotes.

Do prokaryotes have mitochondria?

No, prokaryotes do not have mitochondria. Mitochondria are only found in eukaryotic cells. This is also true of other membrane-bound structures like the nucleus and the Golgi apparatus.
One theory for eukaryotic evolution hypothesizes that mitochondria were first prokaryotic cells that lived inside other cells. Over time, evolution led to these separate organisms functioning as a single organism in the form of a eukaryote.

Eukaryotes are organisms whose cells have a nucleus and other organelles enclosed by a plasma membrane. Organelles are internal structures responsible for a variety of functions, such as energy production and protein synthesis.
Eukaryotic cells are large (around 10-100 μm) and complex. While most eukaryotes are multicellular organisms, there are some single-cell eukaryotes.

Eukaryotic cell features

Within a eukaryotic cell, each membrane-bound structure carries out specific cellular functions. Here is an overview of many of the primary components of eukaryotic cells.

·                     Nucleus: The nucleus stores the genetic information in chromatin form.

·                     Nucleolus: Found inside of the nucleus, the nucleolus is the part of eukaryotic cells where ribosomal RNA is produced.

·                     Plasma membrane: The plasma membrane is a phospholipid bilayer that surrounds the entire cell and encompasses the organelles within.

·                     Cytoskeleton or cell wall: The cytoskeleton or cell wall provides structure, allows for cell movement, and plays a role in cell division.

·                     Ribosomes: Ribosomes are responsible for protein synthesis.

·                     Mitochondria: Mitochondria, also known as the powerhouses of the cell, are responsible for energy production.

·                     Cytoplasm: The cytoplasm is the region of the cell between the nuclear envelope and plasma membrane.

·                     Cytosol: Cytosol is a gel-like substance within the cell that contains the organelles.

·                     Endoplasmic reticulum: The endoplasmic reticulum is an organelle dedicated to protein maturation and transportation.

·                     Vesicles and vacuoles: Vesicles and vacuoles are membrane-bound sacs involved in transportation and storage.

Other common organelles found in many, but not all, eukaryotes include the Golgi apparatus, chloroplasts and lysosomes.

Prokaryote vs eukaryote: key differences

	Prokaryote	Eukaryote
Nucleus	Absent	Present
Membrane-bound organelles	Absent	Present
Cell structure	Unicellular	Mostly multicellular; some unicellular
Cell size	Smaller (0.1-5 μm)	Larger (10-100 μm)
Complexity	Simpler	More complex
DNA Form	Circular	Linear
Examples	Bacteria, archaea	Animals, plants, fungi, protists

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Which of the following is a spherical structure found in the center of animal cell?
a) cell membrane         
b) nucleus
c) cytoplasm                
d) cell wall

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Nucleus constitutes about ……….of the cell’s volume.
a) 10%                        
b) 20%
c) 40%                         
d) 60%

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Which of the following is not a component of the nucleus?
a) Chromosome                       
b) Nucleolus
c) Cytoplasm               
d) Nuclear envelope

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In which of the following cell/s nucleus is absent?
a) uninucleate              
b) enucleate
c) multinucleate                      
d) none of these

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Red blood cells is the example of…………
a) uninucleate              
b) enucleate
c) multinucleate                      
d) none of these

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